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Analysis of Methicillin-Resistant Straphylococcus aureus by Using Randomly Amplified Polymorphic DNA Assay

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KMID : 0647820010240010041
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Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) strains, famous for nosocomical infections, which is an important pathogen of pneumonia, skin infection and bacteremia and account for 5~10% of the whole nosocomical infection. Therefore, knowing these strains transmission and finding their source is a very important thing to know to in order control and to protect us from these strains. If you know a particular transfer route or special environment, one can suppress the transfer and eliminate the cause.
In this study, MRSA were isolated and identificated from ICU, Neu, and Ped environments and we investigated by molecular typing a few MRSA genotypes which used RAPD. In the case of Ped, the MRSA pattern determined the Ia, IIa type, Neu IIa type and the ICU determined the IIa, IIb types. The RAPD method was a very useful technique in molecular typing because the RAPD had the same pattern in the same environment.
KeyWords

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